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{"id":2028,"date":"2026-09-07T11:42:18","date_gmt":"2026-09-07T11:42:18","guid":{"rendered":"https:\/\/www.xiansaishengwu.com\/product\/t7-rna-polymerase\/"},"modified":"2026-09-20T05:30:31","modified_gmt":"2026-09-20T05:30:31","slug":"t7-rna-polymerase","status":"publish","type":"product","link":"https:\/\/www.xiansaishengwu.com\/en\/product\/t7-rna-polymerase\/","title":{"rendered":"T7 RNA\u805a\u5408\u9176"},"content":{"rendered":"

<\/span>\u4ea7\u54c1\u7b80\u4ecb<\/h2>\n
\n

\u672c\u4ea7\u54c1\u662f\u5927\u80a0\u6746\u83cc\u91cd\u7ec4\u8868\u8fbe\u6765\u6e90\u7684\u566c\u83cc\u4f53T7 RNA\u805a\u5408\u9176\uff0c\u4ee5\u542b\u6709T7\u542f\u52a8\u5b50\u5e8f\u5217\uff085\u2019-TAATACGACT CACTATAG*-3\u2019\uff09\u7684\u53cc\u94feDNA\u4e3a\u6a21\u677f\uff0c\u4ee5NTP\u4e3a\u5e95\u7269\uff0c\u5408\u6210\u4e0e\u542f\u52a8\u5b50\u4e0b\u6e38\u7684\u53cd\u5411\u5355\u94feDNA\u4e92\u8865\u7684RNA\u3002\u53cc\u94fe\u7ebf\u6027\u5e73\u672b\u7aef\u62165\u2019\u7a81\u51fa\u672b\u7aefDNA\u5747\u53ef\u4f5c\u4e3aT7 RNA\u805a\u5408\u9176\u7684\u5e95\u7269\u6a21\u677f\uff0c\u56e0\u6b64\u7ebf\u6027\u8d28\u7c92\u3001PCR\u4ea7\u7269\u5747\u53ef\u7528\u4f5c\u4f53\u5916\u5408\u6210RNA\u7684\u6a21\u677f\u3002<\/p>\n<\/div>\n

<\/span>\u4ea7\u54c1\u5e94\u7528<\/h2>\n
\n

1. \u5355\u94feRNA\u5408\u6210\uff08\u5305\u62ecmRNA\uff0csiRNA\uff0cgRNA\u7b49\u5404\u7c7bRNA\u524d\u4f53\uff0c\u4ee5\u53ca\u540c\u4f4d\u7d20\u6807\u8bb0\u6216\u975e\u540c\u4f4d\u7d20\u6807\u8bb0\u7684RNA\u63a2\u9488\uff09<\/p>\n

2. \u4ee5\uff08Cap analog\uff09\u4e3a\u5f15\u7269\u5408\u6210Capped mRNA<\/p>\n<\/div>\n

<\/span>\u4ea7\u54c1\u4f18\u52bf<\/h2>\n
\n
<\/span>T7\u542f\u52a8\u5b50\u9ad8\u5ea6\u7279\u5f02\uff0c\u4ec5\u8f6c\u5f55T7\u542f\u52a8\u5b50\u4e0b\u6e38DNA<\/span><\/div>\n
<\/span>\u8f6c\u5f55\u6548\u7387\u9ad8\uff0c\u9519\u8bef\u7387\u6781\u4f4e<\/span><\/div>\n
<\/span>\u53ef\u5408\u6210capped mRNA<\/span><\/div>\n
<\/span>\u65e0DNase\u3001RNase\u3001\u5207\u53e3\u9176\u6d3b\u6027\u3002<\/span><\/div>\n<\/div>\n

<\/span>\u89c4\u683c\u53c2\u6570<\/h2>\n\n\n\n\n\n\n
\u53c2\u6570<\/th>\n\u8bf4\u660e<\/th>\n<\/tr>\n
\u5206\u5b50\u91cf<\/td>\n\u7ea699 kDa\uff08883\u4e2a\u6c28\u57fa\u9178\uff09<\/td>\n<\/tr>\n
\u6d53\u5ea6<\/td>\n50\u2013200 U\/\u03bcL<\/td>\n<\/tr>\n
\u7f13\u51b2\u6db2<\/td>\n\u542b50 mM Tris-HCl\uff08pH 7.9\uff09\u3001100 mM NaCl\u300120 mM \u03b2-ME\u30011 mM EDTA\u30010.02% Tween 20\u300150%\u7518\u6cb9<\/td>\n<\/tr>\n
<\/td>\n\u5177\u4f53\u4ee5COA\u4e3a\u51c6\u3002<\/td>\n<\/tr>\n<\/table>\n

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